What is lysochrome dye?
A lysochrome is a soluble dye used for histochemical staining of lipids, which include triglycerides, fatty acids, and lipoproteins. Lysochromes such as Sudan IV dissolve in the lipid and show up as colored regions.
How does Sudan IV staining work?
Sudan IV (C24H20N4O) is a lysochrome (fat-soluble dye) diazo dye used for the staining of lipids, triglycerides and lipoproteins on frozen paraffin sections. It has the appearance of reddish brown crystals with melting point 199 °C and maximum absorption at 520(357) nm.
What is the Sudan 3 test?
Sudan III Stain is used to detect fat in feces, urine and tissues. Patients demonstrating fat in stool (i.e., steatorrhea) may have a correlation to pancreatic diseases or other fat absorption diseases.
How do you make a Sudan 3 solution?
Prepare a saturated stock solution of Sudan III in 99% isopropanol. Dilute 6 ml of the stock solution with 4 ml of water. Incubate for 5-10 minutes and then filter the solution. The filtrate can be used for several hours.
What is Sudan IV solution?
How do you make a red O oil solution?
Oil Red O: To make Oil Red O Stock Solution, dissolve Oil Red O in 20 ml of 100% isopropanol, mix well and let it sit for 20 min. Stable for 1 year. To make Oil Red O Working Solution, add 3 parts of Oil Red O Stock Solution to 2 parts of dH2O, mix well, and allow to sit for 10 min.
What is Sudan IV solution used for?
Sudan IV, also called Scarlet red and Fat Ponceau R, is a fat-soluble dye often used for staining triglycerides, lipids and lipoproteins present in cells and tissues.
How do you make a Sudan IV solution?
Solution Prep Use a 0.5% alcoholic solution. Add 0.5 g of sudan IV to 75 mL of warm 96% ethyl alcohol. Dilute ot a final volume of 100 mL with DI water.
What is Sudan solution?
DESCRIPTION. This 15 ml bottle of sudan III stain solution is a biological stain that’s used as a lipid (fat) indicator. Sudan III stains fat cells red, which helps identify the presence of lipids (fats) in liquids and foods.
What color is a negative test for lipids?
Biuret solution is used to test for protein. It is royal blue initially and changes to violet when it reacts with protein. Brown paper bags are used to test for lipids. The paper bag becomes translucent (allows light to pass through) in the presence of lipids.
What is Sudan III solution used for?
This 15 ml bottle of sudan III stain solution is a biological stain that’s used as a lipid (fat) indicator. Sudan III stains fat cells red, which helps identify the presence of lipids (fats) in liquids and foods.
Is Sudan 3 hydrophobic or hydrophilic?
A relatively polar molecule (e.g., acetone, acetonitrile, formamide, methanol, 1-propanol or tetrahydrofuran) can be used as electroosmotic flow marker, and usually a highly hydrophobic, neutral compound such as Sudan III, Sudan IV, dodecanophenone, Orange OT, or Yellow OB as micelle marker.